Official websites use. Share sensitive information only on official, secure websites. Background: Renin-angiotensin system RAS components have been identified in human ciliary body and aqueous humour, pointing to a role for the RAS in the regulation of aqueous humour dynamics.
Here, the authors examine the functional expression of a RAS and the effects of angiotensin II AII on a signal transduction pathway and ion secretion mechanism in cultured human ciliary body non-pigmented epithelium HNPE. Single channel patch clamp techniques were employed to investigate ion channel activity. P o increased from 0. A simultaneous cell volume reduction of These effects are accompanied simultaneously by cell volume loss, indicating that AII acts as receptor operated secretagogue in HNPE cells.
The ability of an AT 1 receptor antagonist to inhibit these processes may thus offer a new family of pharmaceutical agents to the current armamentarium in the treatment of glaucoma. Keywords: renin-angiotensin system, secretory function, ciliary body, epithelium. The circulating renin-angiotensin system RAS , through the production of its biological effector angiotensin II AII , has an important role in the control of electrolyte homeostasis, renal haemodynamics, and blood pressure.
In humans, prorenin, the inactive precursor of renin, has been detected in ciliary body tissue 5, 6 and the presence of angiotensin converting enzyme has been shown in ciliary body, vitreous, and aqueous humour. A human non-pigmented ciliary body epithelial cell line HNPE has been established by transfection with simian virus The ability of AII to trigger secretion is indicated through its effects on potassium ion channel activation and cell volume reduction. The cell clone ODM Cl-2 was established from a primary culture of human non-pigmented ciliary epithelium.
The medium contained mg per litre, d -glucose and l -glutamine. NaHCO 3 was added to the medium to adjust the pH to between 7. The medium was sterilised by membrane filtration with 0.
